A Novel Method to Radiolabel Stealth Liposome through 1,2- dimyristoyl-sn-glycero-3-phosphoethanolamine-N-DTPA with 99mTc and Biological Evaluation

• Mirel Cabrera

  Arera de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República. Mataojo 2055, 11400 Montevideo, Uruguay

• Alejandra Medrano

  Departamento de Ciencia y Tecnología de Alimentos, Facultad de Química, Universidad de la República. General Flores 2124, 11800, Montevideo, Uruguay

• Nicole Lecot

  Arera de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República. Mataojo 2055, 11400 Montevideo, Uruguay

• Marcelo Fernandez

  Arera de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República. Mataojo 2055, 11400 Montevideo, Uruguay

• Maria Moreno

  Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República. Av.Alfredo Navarro 3051, 11600, Montevideo, Uruguay

• Jose A Chabalgoity

  Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República. Av.Alfredo Navarro 3051, 11600, Montevideo, Uruguay

• Juan Pablo Gambini

  Centro de Medicina Nuclear, Hospital de Clínicas “Dr. Manuel Quintela”, Facultad de Medicina, Universidad de la República. Av. Italia s/n, 11600, Montevideo, Uruguay

• Omar Alonso

  Centro de Medicina Nuclear, Hospital de Clínicas “Dr. Manuel Quintela”, Facultad de Medicina, Universidad de la República. Av. Italia s/n, 11600, Montevideo, Uruguay

• Henia Balter

  Arera de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República. Mataojo 2055, 11400 Montevideo, Uruguay

• Pablo Cabral

  Arera de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República. Mataojo 2055, 11400 Montevideo, Uruguay

Purpose: To study surface technetium labeling of stealth DTPA-Liposomeand to evaluate its potential as a molecular imaging tracer for both normal and melanoma-bearing mice.

The radiolabeling yield of liposomes was greater than 90%and showed good chemical and biological stability. Biodistribution studies in normal mice showed blood clearance with hepatic and renal depuration. Melanoma-bearing mice showed a similar pattern of biodistribution with high tumor uptake allowing tumor imaging.

The developed method of surface radiolabeled DTPA-PEG-Liposomes with ^99mTc was effective and stable in vivo.

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