MiR-130b-3p Suppress the Migration, Proliferation and Chemosensitization of Hepatocellular Carcinoma Cells

Teoh Han  Pinn; Siti Fathiah  Masre; Nadiah  Abu

doi:10.30683/1929-2279.2024.13.04

Authors

Teoh Han Pinn Department of Biomedical Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
Siti Fathiah Masre Department of Biomedical Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
Nadiah Abu UKM Medical Molecular Biology Institute (UMBI), Universiti Kebangsaan Malaysia, 56000 Kuala Lumpur, Malaysia

DOI:

https://doi.org/10.30683/1929-2279.2024.13.04

Keywords:

HCC, small RNA, non-coding RNA, HepG2, small RNAs, mir130b

Abstract

Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed cancers globally, yet its pathogenesis remains incompletely understood. Among the various mechanisms contributing to HCC development, small RNAs, such as microRNAs (miRNAs), play a significant role. miRNAs are non-coding RNAs, typically 20-30 nucleotides long, that regulate gene transcription by binding to RNAs, affecting downstream signaling pathways. One such miRNA, hsa-miR-130b-3p, has been associated with cancer development, including HCC, although the full extent of its involvement remains unclear. This study aimed to explore the link between hsa-miR-130b-3p and HCC using bioinformatics analyses and in vitro assays. Publicly available databases were utilized for expression profiling, mRNA and lncRNA target prediction, pathway enrichment, and methylation analysis. In vitro experiments were conducted using a hsa-miR-130b-3p inhibitor in HepG2 cells to assess its effects on proliferation, migration, and oxaliplatin sensitivity. Our findings show that hsa-miR-130b-3p is upregulated in multiple cancers, including HCC, targeting cancer-related genes and interacting with various lncRNAs. Inhibition of hsa-miR-130b-3p reduced cancer cell proliferation and migration, while enhancing drug sensitivity to oxaliplatin. These results suggest that hsa-miR-130b-3p may play a role in HCC pathogenesis, but further studies are required to fully understand its mechanisms.

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